The effect of different glycerol concentrations on freezability of semen from Angora, Kilis and Saanen goats
2013
Kulaksiz, R., Kafkas University, Kars (Turkey) | Ari, Ç., Kafkas University, Kars (Turkey) | Daşkin, A., University of Ankara, Ankara (Turkey) | Üner, A. G., University of Adnan Menderes, Aydın (Turkey)
The aim of this study was to investigate effect of different glycerol concentrations on freezability of semen from Angora, Kilis and Saanen goats. Three male goats from each breed were selected and ejaculates were collected with artificial vagina. Three ejaculates from each breed were pooled and extended with skim milk-based extender containing 10 % (v/v) egg yolk and 0 %, 3 %, 5 %, 7 % and 9 % (v/v) glycerol (G0, G3, G5, G7 and G9, resp.) as a cryoprotectant. Extended semen from different goat breeds was equilibrated, cryopreserved and stored in liquid nitrogen. The best post-thaw motility for Angora (51.6 %) and Kilis (75.0 %) goats was obtained with G5 concentration, while the best post-thaw motility for Saanen goat (61.6 %) was obtained with G7 concentration (P less than 0.001). Similar results were recorded for percentage of live spermatozoa for Angora (58.1 % in G5), Kilis (78.5 % in G5) and Saanen (64.0 % in G7) goats (P less than 0.001). The lowest abnormal spermatozoa percentages were obtained with G5 concentration for Angora (34.3 %), Kilis (30.8 %) and Saanen (40.8 %) goats (P less than 0.001). While different glycerol concentrations for goat breeds were considered, it was determined that suitable glycerol percentages for Angora, Kilis and Saanen goats were 5 %, 5-9 % and 7 % respectively. It was concluded that glycerol concentration is an important factor affecting freezability of goat semen from different breeds.
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