Genetic analysis of apple from Guizhou Province rootstock germplasms using ISSR markers | 贵州苹果砧木资源亲缘关系的ISSR分析
2013
Song Sha, Institute of Fruit Tree of Guizhou Academy of Agricultural Sciences, Guizhou (China) | Wu Yawei, Guizhou University, Guiyang (China), Key Laboratory of Agricultural Bioengineering at Guizhou Province | Ji Xiangbiao, Guizhou University, Guiyang (China), College of Life Science
chinois. 利用ISSR标记，对原产于贵州的10份苹果砧木种质及5份外来资源进行亲缘关系分析。结果表明：采用筛选出的10条引物，共扩增出75条重复性好的谱带，其中67条（占89．3％）具有多态性；应用引物827、M05、M08可以有效区分出15份种质资源，构建其DNA的ISSR指纹图谱；聚类分析表明，供试种质间的遗传相似系数在0.49～0.93之间，贵州威宁苹果砧木资源遗传多样性较为丰富，ISSR分析结果与已知苹果砧木间的谱系较为一致。
Afficher plus [+] Moins [-]anglais. The genetic relationship of 15 apple rootstock germplasms were studied using ISSR markers. Ten pairs of primers were used and a total of 75 bands were yielded, among which 67 were polymorphic. Primers 827, M05 and M08 could be used for effectively genotyping the 15 germplasms. The systematical cluster analysis showed that 15 apple rootstock germplasms could be divided into three groups. Malus, Pyrus and Sorbus with a genetic similarity coefficient of 0.66, which fitted well with the morphological genealogy.
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