Effects of spraying exogenous YCL2 and Z5 on nicotine content and two key enzymes regulating nicotine synthesis in burley tobacco | 喷施外源植物生长调节物质对白肋烟烟碱含量及其合成关键酶活性的影响

chinois. 采用盆栽试验，研究白肋烟打顶后喷施外源植物生长调节物质YCL2和Z5对烟碱含量及其合成关键酶（鸟氨酸脱羧酶和精氨酸脱羧酶）的活性以及总氮、总糖、氯和钾含量的影响。结果表明：白肋烟打顶后喷施YCL2和Z5能够显著降低白肋烟叶片烟碱的含量，其中喷施YCL2使上部叶片烟碱含量降低20.47%，喷施Z5使上、中部叶片烟碱含量分别降低34.36%和32.38%；喷施YCL2和Z5能显著降低合成烟碱关键酶（鸟氨酸脱羧酶和精氨酸脱羧酶）的活性。喷施YCL2和Z5对烟叶重要品质指标（总氮、总糖、氯和钾）的含量没有显著影响。结果说明，喷施外源植物生长调节物质YCL2和Z5能显著降低烟叶烟碱含量，但不影响烟叶品质，在烟叶生产调控烟碱含量上具有较大的推广应用价值。

anglais. A pot experiment was carried out to study the effect of spraying exogenous substances YCL2 and Z5 after topping on the nicotine content, enzymatic activity of arginine decarboxylase and ornithine decarboxylase regulating the metabolism of nicotine, and total nitrogen, total sugar, chlorine, potassium. The results showed that spraying YCL2 and Z5 could significantly reduce the nicotine content with few influence on the other chemical qualities. With application of exogenous substance reducing the activity of arginine decarboxylase and ornithine decarboxylase, YCL2 decreased nicotine content by 20.47% in upper leaves, and Z5 decreased 34.36% and 32.38% in upper and middle leaves, respectively. It indicated that the effect of spraying YCL2 and Z5 on reducing nicotine content may be significantly related with its roles in decreasing the activity of arginine decarboxylase and ornithine decarboxylase. It would be one potential pathway to control nocotine content in burley tobacco production.