Detection Method for Identification of Pueraria mirifica (Thai kudzu) in Processed Foods
2012
Park, Y.C., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Jin, S.W., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Kim, M.R., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Kim, K.H., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Lee, J.H., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Cho, T.Y., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Lee, H.J., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Lee, S.J., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea | Han, S.B., National Institute of Food and Drug Safety Evaluation, Cheongwon, Republic of Korea
In this study, ribulose bisphosphate carboxylase (rbcL), RNApolymeraseC (rpoC1), intergenic spacer (psbA-trnH), and second internal transcribed spacer (ITS2) as identification markers for discrimination of P. mirifica in foods were selected. To be primer design, we obtained 719 bp, 520 bp, 348 bp, and 507 bp amplicon using universal primers from selected regions of P. mirifica. The regions of rbcL, rpoC1, and psbA-trnH were not proper for design primers because of high homology about P. mirifica, P. lobata, and B. superba. But, we had designed 4 pairs of oligonucleotide primers from ITS2 gene. Predicted amplicon from P. mirifica were obtained 137 bp and 216 bp using finally designed primers SFI12-miri-6F/SFI12-miri-7R and SFI12-miri-6F/SFI12-miri-8R, respectively. The species-specific primers distinguished P. mirifica from related species were able to apply food materials and processed foods. The developed PCR method would be applicable to food safety management for illegally distributed products in markets and internet shopping malls.
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