Enhanced Production of Endo-b-1, 4-xylanase from Paenibacillus sp. HX-1 newly Isolated from soil Samples at Hambak Mountain in Yongin city, Korea
2013
Chi, W.J., Myongji University, Yongin, Republic of Korea | Kim, J.H., Seoil College, Seoul, Republic of Korea | Hong, S.K., Myongji University, Yongin, Republic of Korea
A xylanase-producing bacterium was isolated from a soil sample collected in Yongin city, Korea. The strain was aerobic and gram positive, and grew between pH 5.0 and 11.0, forming a yellow-colored colony. The strain was classified as a novel subspecies bacterium of Paenibacillus barcinonensis by 16S rRNA gene sequence similarity, phylogenetic analysis, phenotypic, and biochemical characteristics, and thus named Paenibacillus sp. HX-1. This strain produced extracellular endo-β-1,4-xylanase, and the best xylanolytic activity (205.17 unit/ml) was obtained at 96 h in an optimized TNX medium containing 1% (w/v) bacto tryptone, 1% (w/v) NaCl, and 0.7% (w/v) beechwood xylan at pH 7.0, 37℃ and 200 rpm. The endo-β-1,4-xylanase produced by the strain HX-1 yielded xylobiose as the end product from beechwood xylan hydrolysis. The enzyme exhibited optimum pH and temperature at pH 7.0 and 45℃, respectively. The remarkable enhancing effect of the TNX medium on xylanase production by HX-1, in spite of its simple formula, may have great advantages for industrial applications of xylanase.
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