Measurement of Density for Xanthomoanas oryzae pv. oryzae Cell Using Real Time PCR
2013
Noh, T.H., NICS, Iksan, Republic of Korea | Kang, M.H., RDA, Suwon, Republic of Korea | Seo, S.J., NICS, Iksan, Republic of Korea | Shim, H.K., NICS, Iksan, Republic of Korea | Choi, M.Y., NICS, Iksan, Republic of Korea | Park, J.C., NICS, Iksan, Republic of Korea | Back, C.H., RDA, Suwon, Republic of Korea | Kim, H.M., National University, Jeonju, Republic of Korea
We developed a new measurement method for the density of Xanthomonas oryzae pv. oryzae(Xoo), a casual pathogen of bacterial leaf blight of rice. The new method used real time PCR with the highly characterized and specific Xan_PahgeF and Xan_PahgeR primer and Xan_Pahge FAM MGB probe primer pairs derived from phage-related integrase gene. The method was effective when tested with a variety of templates including isolated Xoo DNA, pure colonies or liquid culture sources, and could also work on contaminating water. A main advantage of this assay is to accurately and rapidly quantitate the population density of Xoo in paddy field water containing inhibitors.
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