Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection
2013
Kim, K., Kangwon National University, Chuncheon, Republic of Korea | Kim, D., Kangwon National University, Chuncheon, Republic of Korea | Sun, J., Kangwon National University, Chuncheon, Republic of Korea | Park, S., Kangwon National University, Chuncheon, Republic of Korea | Cho, Y., Kangwon National University, Chuncheon, Republic of Korea | Ko, H.J., Kangwon National University, Chuncheon, Republic of Korea | Joo, H.G., Jeju National University, Jeju, Republic of Korea | Hahn, T.W., Kangwon National University, Chuncheon, Republic of Korea
The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.
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