Difference in Growth, Phenolics Content and Antioxidant Activity of Cowpea Sprouts at Different Plant Parts
2013
Chon, S.U., EFARINET Co. Ltd., Gwangju, Republic of Korea
An experiment was conducted to determine the content of phenolics and flavonoids, antioxidant activity and antioxidant enzyme status for the extracts from 5 and 7-day old sprouts (DOS) of cowpea (Vigna unguiculata L. Walp). Total phenolics [mg ferulic acid equivalents (FAE) kg-1 DW] content was highest in cotyledon extracts (48.8 mg kg-1), followed by roots (30.8 mg kg-1) and hypocotyl (22.2 mg kg-1) extracts (p 0.05) from 5 DOS. The result of total flavonoid level [mg rutin equivalents kg-1 DW] had same tendency to the results of total phenolics, showing lower amount ranges. The antioxidant activity of the methanol extracts from all the plant dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was higher in cotyledon extracts (82.5%) than in root (52.6%) or hypocotyl parts (35.0%) from 5 DOS. Among antioxidant enzymes, APX and CAT activities were highest in cotyledon part and POX and SOD activities in root part of 5 and 7 DOS. The results showed that total phenolics content (r2
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