Study on Contents Comparison of Foenumoside B in Different Parts of Lysimachia foenum-graecum
2014
Um, Y.R., Pharmaceutical R and D center, Kolmar Korea Co., Ltd, Sejong, Republic of Korea | Mok, S.Y., Pharmaceutical R and D center, Kolmar Korea Co., Ltd, Sejong, Republic of Korea | Hyuck, S.J., Pharmaceutical R and D center, Kolmar Korea Co., Ltd, Sejong, Republic of Korea | Kim, S.K., Pharmaceutical R and D center, Kolmar Korea Co., Ltd, Sejong, Republic of Korea | Cho, B.H., Pharmaceutical R and D center, Kolmar Korea Co., Ltd, Sejong, Republic of Korea | Cho, Y.B., Pharmaceutical R and D center, Kolmar Korea Co., Ltd, Sejong, Republic of Korea
Lysimachia foenum-graecum (LF) has been used for home remedy of common cold and headache in China. Foenumoside B from LF has been reported for anti-obesity effects. We used foenumoside B as a marker for content evaluation of different parts of LF. Ethanol extract of LF was used for isolation of foenumoside B and purified further by column chromatography. The structure was identified as foenumoside B by interpretation of spectroscopic analysis, including ©�H -, ©�©�C -NMR and FT-ICR-MS. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of foenumoside B in different parts of LF. Foenumoside B contents in the leaf, stem, and aerial part showed significant differences. Contents of foenumoside B was detected highly in the leaf extracts. The results would be useful for efficient extraction of foenumoside B in LF.
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