Antioxidant and α-Glucosidase Inhibition Activities of Solvent Fractions from Methanolic Extract of Sericea Lespedeza (Lespedeza cuneata G. Don)
2012
Kim, H.Y., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Ko, J.Y., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Song, S.B., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Kim, J.I., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Seo, H.I., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Lee, J.S., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Kwak, D.Y., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Jung, T.W., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Kim, K.Y., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Oh, I.S., National Institute of Crop Science, RDA, Milyang, Republic of Korea | Jeong, H.S., Chungbuk National University, Cheongju, Republic of Korea | Woo, K.S., National Institute of Crop Science, RDA, Milyang, Republic of Korea
The purpose of this study was to evaluate the antioxidant contents and antioxidant activities of solvent fractions from methanolic extract of sericea lespedeza. To determine the antioxidant compounds in solvent fractions from methanolic extract, total polyphenolic, flavonoid, tannin, and proanthocyanidin contents were measured by spectrophotometric methods. Solvent fractions were evaluated for antioxidative capacity according to DPPH and ABTS radical scavenging activities. Total polyphenolic contents were 12.44, 3.61, 6.39, 27.11, 20.00, and 9.32 mg gallic acid equivalent (GAE)/g extract residue (ER), respectively. Total flavonoid contents were 2.94, 9.92, 7.77, 9.27, 5.11, and 2.66 mg catechin equivalent (CE)/g ER, respectively. Total tannin contents were 8.75, 10.04, 7.42, 17.32, 11.65, and 7.61 mg tannic acid equivalent (TAE)/g ER, respectively. Total proanthocyanidin contents were 346.09, 63.50, 103.76, 288.62, 231.99, and 358.48 ㎍ CE/g ER, respectively. DPPH radical scavenging activities of solvent fractions from methanolic extract of sericea lespedeza were 20.62, 5.16, 9.29, 20.80, 20.00 and 20.79 mg Trolox equivalent (TE)/g ER, and ABTS radical scavenging activities were 33.86, 9.24, 17.36, 33.76, 33.49, and 33.86 mg TE/g ER, respectively. SOD-like activities were 4.12, 0.61, 2.01, 9.89, 13.47, and 11.82 units/mL, and α-glucosidase inhibition activities were 93.85 and 61.64% at concentrations of 50 and 25 ㎍/mL in the water fraction, respectively. The results of this study show that notable antioxidant activities in sericea lespedeza have significant health benefits.
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