Phytoplasmoses en arboriculture fruitière: diagnostic par PCR en temps reel ou par PCR nichée? | Phytoplasma-related diseases in fruit growing: diagnosis by real time PCR or by nested PCR?
2014
Johnston, H., Agroscope Changins-Waedenswil (ACW), Nyon (Switzerland) | Genini, M. | Buenter, M. | Schaerer, S.
Nested PCR and real time PCR are routinely used techniques for the diagnosis of phytoplasma-related diseases in fruit growing. The first variant, used in Agroscope’s laboratory of phytoplasmology, in Changins, was compared to the second variant, using one hundred and sixteen apple, pear and apricot trees. The test shows that real time PCR is more accurate than nested PCR, but marginally so. This small difference may have significant impact on the production of certified fruit plant material. However, the main factor determining the extent of the imprecision appears to be linked to the type of sampling chosen.
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