Direct identification of bifidobacteria from probiotic supplements
2014
Bunesova, V., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Microbiology, Nutrition and Dietetics | Vlkova, E., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Microbiology, Nutrition and Dietetics | Rada, V., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Microbiology, Nutrition and Dietetics | Hovorkova, P., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Microbiology, Nutrition and Dietetics | Rockova, S., Czech University of Life Sciences, Prague (Czech Republic). Dept. of Microbiology, Nutrition and Dietetics | Kmet, V., Slovak Academy of Sciences, Kosice (Slovak Republic). Institute of Animal Physiology
The DNA of 14 probiotic supplements was isolated directly from various products without previous cultivation. The bifidobacteria composition declared by the manufacturer was determined by species-specific PCR. Such species found were in accordance with those listed on the products. This approach can be performed in less than 5 h and is applicable to other genera of probiotic bacteria. Bifidobacteria were also detected by culture-dependent analysis and MALDI-TOF MS. These methods drew the same results; however, they are more expensive and time consuming. So, we propose direct identification of bifidobacteria for routine quality control inspections of species composition in probiotic supplements.
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