Yeast with amylolytic, lipolytic and preteolytic activities isolated from mango (Magnifera indica L.)fruits
2014
Tan Gana, N.H. | Mendoza, B.C. | Monsalud, R.G.
Yeasts were isolated from mangoes, an untapped resource for yeasts bioprospecting. Fruit washings from three varieties of ripe healthy mangoes, namely: 'Kalabaw', 'Indian' and 'Piko' were spread plated onto yeast extract malt extract agar (YMA) and ethanol sulfide agar (ESA) supplemented with streptomycin and biphenel. Two hundred seventeen yeasts were isolated from six mango samples. The isolates were screened for amylase, lipase and protease on starch agar, Sierra's medium with Tween 80 and casein agar, respectively. Yeasts that produced lipase numbered 100 while 51 produced amylase and 46 produced protease. Phenotypic identification of 29 yeasts which had moderate to high enzymatic activities was done and 11 were subjected to D1/D2 26S ribosomal RNA gene (D1/D2 26S rRNA) sequencing. The yeasts with amylolytic, lipolytic and/or proteolytic activities which where identified include: Aureobasidium pullulans var. melanogenum, Aurobasidium sp., Candida haemulonii, Cryptococcus aerius, C. flavescens, Cryptococus sp. and Rhodosparidium paludigenum. This study provided a snap shot of the diversity of the yeast microflora in the surface of ripe healthy mangoes from the Philippines and it has identified and preserved enzyme-producing yeast strains with possible commercial use.
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