Latgales meloņu klonēšana in vitro | The in vitro cloning of Latgales melon’s
2016
Halzovs, J., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Agriculture;Pure Horticultural Research Centre, Pure parish, Tukuma Municipality (Latvia) | Vasiljevs,G., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Agriculture | Alsina, I., Latvia Univ. of Agriculture, Jelgava (Latvia). Faculty of Agriculture
Melon (Cucumis melo L.) is an important horticultural crop. To contribute melon growth in Latvia, it is necessary to provide proper planting material. Valuable genetic material of Latvia’s melons has been obtained from Pure Horticultural Research Centre. The obtained genetic material was propagated from seeds of two seasons from 2014 to 2015 in Botanical Garden of Latvia University on opened field. Latgale`s melon lines 4(3), 5(2), 8, 14 were introduced from seeds in Murashige and Skoog medium and cloned two times. The main objective was to observe the morphological evolution and characteristics of Latgale`s melon clones in vitro. This method is particularly important for genetically homogenous clones reproducing for breeding needs. Line 4(3) of Latgale’s melon seeds showed the best results of all the other lines, and 2 of 10 seeds germinated. The seeds of Latgale`s melon lines 8 and 5(2) in vitro did not germinate. The more productive clone was the line 14 by releasing the 31 clone from one seed. The Latgale’s melon clones reproduce the roots without auxins and cytokinins added to the culture medium.
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