Some physiological characteristics of the two pathogenic fungi from Ophiostoma genus
2012
Golubović-Ćurguz, Vesna (Faculty of Forestry, Belgrade (Serbia)) | Karadžić, Dragan (Faculty of Forestry, Belgrade (Serbia))
This paper presents the results of the study of the same phisiological characteristics of the two species of pathogenic fungi from Ophiostoma genus (Ophiostoma piceae (Munch) Syd. & P.Syd., and Ophiostoma ulmi (Buisman)Nannf). We have tested the influence of different temperatures and different nutritive media to the mycelia growth of this fungus, than fungal fermenting activity, firstly, production of oxidase and reductase. In the laboratory conditions we tesed the effect of different temperatures (6C, 10C, 11C, 17C, 18C, 20C, 23C, 24C, 26C, 27C, 31C) and two different media: Malt Extract Agar (MEA) and selective media (MEA with actidion and streptomycin) on the growth rate and morphological shape of mycelium of fungi. The fungal fermenting activity was analyzed by testing the influences of isolated fungi on the media oxidation degree. According to the results of our test fungi exhibited physiological activity at temperatures ranging from 6C to 31C, while the best conditions for growth of mycelium in the temperature range from 20C to 27C. Our results show that better daily and weekly mycelial growth of O. picea was recorded on MEA nutritive medium, while O. ulmi had better growth on a selective medium MEA. On media with gallic and tannic acid isolates showed slower growth, but the recorded oxidation of these substrates in all the samples.
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