The application of RNA interference libraries to functional genomics studies in mammals.

Shen He; Fan Bin; Zhao ShuHong

The application of RNA interference libraries to functional genomics studies in mammals.

2010

Shen He | Fan Bin | Zhao ShuHong

RNA interference (RNAi) technology is a gene-silencing tool with high specificity and efficiency for gene function studies. The use of RNAi libraries, which is based on this technology, promotes the significance of RNAi in gene silencing, and furthermore it provides a basis for the large-scale gene function analyses and high-throughput gene scan studies. This paper reviews the several types of RNAi library preparation, their respective construction approaches and some critical issues during RNAi library construction.

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Mots clés AGROVOC

gene silencing genes genomics mammals rna

Informations bibliographiques

Publié dans

CAB Reviews

Volume 5 Numéro 001

Editeur

CABI

Pagination

1-9

D'autres materias

Rna interference

Langue

anglais

Note

Author Affiliation: Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Shisishan Road No. 1, Hongshan District, Wuhan 430070, China.

Titre traduit

CAB Reviews

Sur AGRIS depuis: 2018-10-15

Format: AGRIS AP

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Liens

DOI http://sites.cabi.org/review/20103129288

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