Antioxidant, anti-inflammatory, and anti-pruritic effects of grape branch extract
2016
Cho, B.O., Research Institute, Ato Q and A Corporation, Jeonju, Republic of Korea | Yin, H.H., Research Institute, Ato Q and A Corporation, Jeonju, Republic of Korea | Che, D.N., Research Institute, Ato Q and A Corporation, Jeonju, Republic of Korea | Kim, S.J., Jeonju AgroBio-Materials Institute, Jeonju, Republic of Korea | Ryu, C., Hyangmiwon Corporation, Gimje, Republic of Korea | Jang, S.I., Research Institute, Ato Q and A Corporation, Jeonju, Republic of Korea
This study was designed to investigate the antioxidant activities of the ethanol extracts (GBE) of grape branches (Campbell Early). The total polyphenol and flavonoid content of GBE was 201.42±4.16 and 11.85±0.44 mg GAE/g, respectively. The antioxidant activity of GBE was measured using the ABTS and DPPH assays, and the IC50 values were 45.60±0.09 and 299.13±0.22, respectively. GBE inhibited the production of pro-inflammatory mediators (NO, iNOS, PGE2, COX-2, IL-1beta, and IL-6) in lipopolysaccharide-stimulated RAW 264.7 macrophages in a dose-dependent manner. Moreover, GBE treatment significantly suppressed the production of TNF-alpha and IL-6 cytokines in phorbol 12- myristate 13-acetate plus calcium ionophore A23187-stimulated HMC-1 human mast cells. Furthermore, the administration of GBE markedly inhibited the scratching behavior induced by the compound 48/80 in ICR mice. These results suggested that GBE has potential as a therapeutic agent against inflammation and itch-related skin diseases.
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