Effect of Liquid Culture on the Propagation of Virus free Plantlets from Meristem Culture-derived of Sweetpotato [Ipomoea batatas L. (Lam)]
2016
Nam, S.S., National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea | Chung, M.N., RDA, Wansan, Republic of Korea | Lee, J.S., National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea | Lee, H.U., National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea | Yang, J.W., National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea | Hwang, E.J., National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea | Lee, K.B., National Institute of Crop Science, Rural Development Administration, Muan, Republic of Korea
The most common method of vegetative propagation of virus free plantlets is the use of shoots meristem culture in solid medium culture. This study was to investigate the effect on liquid medium culture for the growth of virus-free sweetpotato plantlets. Single-nodes derived from meristem culture of sweetpotato was examined in this experiment and three sweetpotato varieties Singeonmi, Sinhwangmi, and Sinjami was used. The growth of plnatlets was greater in liquid medium culture than that of solid medium culture after longer incubation in 3 varieties. The total fresh weight of 5 week old plantlets after planting in solid culture were 2.17 g (Singeonmi), 2.49 g (Sinhwangmi), and 2.18 g (Sinjami), but the fresh weight in liquid medium culture was 3.87, 3.88, and 3.35 g, respectively. Leaf number of Singeonmi and Sinjami plantlets after 5 weeks of liquid medium culture was 21.1 and 22.6, respectively and liquid medium culture showed 3 and 6.2 more leaf number than that of solid medium culture. Plant height of Sinhwangmi and Sinjami plantlets after 3 weeks of liquid medium culture was 4.1 cm and 3.4 cm, respectively, and liquid medium culture showed 1.1 cm longer stem length than solid medium. Overall, liquid medium culture of sweetpotato plantlets was more effective than solid medium in terms of leaf and stem growth.
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