Development and Validation of an Analytical Method for Determination of Fungicide Benzovindiflupyr in Agricultural Commodities Using LC-MS/MS
2017
Lim, S.H., Ministry of Food and Drug Safety, Cheongju, Republic of Korea | Do, J.A., Ministry of Food and Drug Safety, Cheongju, Republic of Korea | Park, S.M., Ministry of Food and Drug Safety, Cheongju, Republic of Korea | Pak, W.M., Ministry of Food and Drug Safety, Cheongju, Republic of Korea | Yoon, J.H., Ministry of Food and Drug Safety, Cheongju, Republic of Korea | Kim, J.Y., Import Food Analysis Division, Seoul Regional Food and Drug Administration, Seoul, Republic of Korea | Chang, M.I., Audit and Inspection Office, Ministry of Food and Drug Safety, Cheongju, Republic of Korea
Benzovindiflupyr is a new pyrazole carboxamide fungicide that inhibits succinate dehydrogenase of mitochondrial respiratory chain. This study was carried out to develop an analytical method for the determination of benzovindiflupyr residues in agricultural commodities using LC-MS/MS. The benzovindiflupyr residues in samples were extracted by using acetonitrile, partitioned with dichloromethane, and then purified with silica solid phase extraction (SPE) cartridge. Correlation coefficient(r²) of benzovindiflupyr standard solution was 0.99 over the calibration ranges(0.001~0.5 μg/mL). Recovery tests were conducted on 5 representative agricultural commodities (mandarin, green pepper, potato, soybean, and hulled rice) to validate the analytical method. The recoveries ranged from 79.3% to 110.0% and then relative standard deviation (RSD) was less than 9.1%. Also the limit of detection (LOD) and limit of quantification (LOQ) were 0.0005 and 0.005 mg/kg, respectively. The recoveries of interlaboratory validation ranged from 83.4% to 117.3% and the coefficient of variation (CV) was 9.0%. All results were followed with Codex guideline (CAC/GL 40) and Ministry of Food and Safety guideline (MFDS, 2016). The proposed new analytical method proved to be accurate, effective, and sensitive for benzovindiflupyr determination and would be used as an official analytical method.
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