Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle
2017
Kim, N., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Jang, Y., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kim, J.K., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Ryoo, S., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kwon, K.H., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kim, M., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Kang, S.S., Chungcheongbukdo Veterinary Service, Chungju, Republic of Korea | Byeon, H.S., Chungcheongbukdo Veterinary Service, Chungju, Republic of Korea | Lee, H.S., Animal and Plant Quarantine Agency, Anyang, Republic of Korea | Lim, Y.H., Korea University, Seoul, Republic of Korea | Kim, J.M., Animal and Plant Quarantine Agency, Anyang, Republic of Korea
The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.
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