Overexpression of Brassica rapa GROWTH-REGULATING FACTOR genes in Arabidopsis thaliana increases organ growth by enhancing cell proliferation
2017
Hong, J.K., National Institute of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea | Oh, S.W., National Institute of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea | Kim, J.H., Kyungpook National University, Daegu, Republic of Korea | Lee, S.B., National Institute of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea | Suh, E.J., National Institute of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea | Lee, Y.H., National Institute of Agricultural Science, Rural Development Administration, Jeonju, Republic of Korea
GROWTH-REGULATING FACTOR(GRF) genes encodeplant-specific transcription factors containing two conserved QLQ and WRC domains and play criticalroles inregulating the growth and development of lateral organs, such as cotyledons, leaves, and flowers. Toexplore the agricultural potential of Brassica rapa GRF genes(BrGRFs), the researchersisolatedseven BrGRFs(BrGRF3-1, 3-2, 5, 7, 8-1, 8-2, and 9)and constructed BrGRF-overexpressing Arabidopsis thaliana plants (BrGRF-OX). BrGRF-OXplants developed larger cotyledons, leaves, andflowers as well as longer rootsthan the wild type. The increase in size of these organs were due to increases in cell number, but not due to cell size. BrGRF-OXplants alsohad larger siliquesand seeds. Furthermore, BrGRF-OXseeds produced more oil than the wild type. RT-PCR analysis revealed that BrGRF sregulatedexpression of a wide range ofgenes thatare involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, thedata indicates that BrGRF sact aspositive regulators of plant growth, thus raising the possibility thatthey may serve as a useful genetic source for crop improvement with respect toorgan size and seed oil production.
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