Functional Characterization of Thioredoxin h Type 5 with Antimicrobial Activity from Arabidopsis thaliana
2017
Park, S.C., Sunchon National University, Suncheon, Republic of Korea | Jung, Y.J., National Institute of Ecology, Seocheon, Republic of Korea | Kim, I.R., National Institute of Ecology, Seocheon, Republic of Korea | Lee, Y., Texas A and M University, USA | Kim, Y.M., Sunchon National University, Suncheon, Republic of Korea | Jang, M.K., Sunchon National University, Suncheon, Republic of Korea | Lee, J.R., National Institute of Ecology, Seocheon, Republic of Korea
A plant protein with effective antifungal activity was isolated from Arabidopsis thaliana leaves. The results of N-terminal sequencing analysis revealed that the isolated protein matches with a partial fragment of A. thaliana oxidoreductase thioredoxin h type 5; AtTrx-h5 (GenBank accession number AT1G45145). The AtTrx-h5 gene isolated from an Arabidopsis leaf cDNA library was transformed into Escherichia coli to investigate the functional characterizations of AtTrx-h5 protein. Recombinant AtTrx-h5 protein inhibited the conidia germination in 7 filamentous fungal cells and the proliferation in 3 pathogenic yeast cells. In addition, the cellular distribution of recombinant AtTrx-h5 protein in Fusarium solani showed pH-dependent cytosolic accumulation. Interestingly, AtTrx-h5 acted as an inhibitor of fungal growth via cellular reactive oxygen species (ROS) generation. We propose the novel functions of AtTrx-h5 as a potential candidate for natural antifungal material in the study.
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