Quantitative Analysis and Anti-inflammatory of Lyoniside from the Pourthiaea villosa var. brunnea
2016
Woo, K.W., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea | Seong, T.K., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea | Lee, H.M., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea | Jang, J.H., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea | Lee, K.H., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea | Cho, H.W., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea | Cho, J.H., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea | An, B., Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine, Jangheung, Republic of Korea
In this study, the purification of the MeOH extract from the stems and leaves of Pourthiaea villosa var. brunnea using column chromatography furnished a main compound, lyoniside. The structure was elucidated on the basis of 1 H and 13 C NMR spectroscopic data. Quantitative analysis of lyoniside was conducted by HPLC method and the highest content of lyoniside was found in 50% MeOH reflux extraction. To investigate the anti-inflammatory effect of the lyoniside, we measured nitric oxide and pro-inflammatory cytokines such as TNF-alpha, IL-1beta, IL-6 levels in lipopolysaccharide-induced murine macrophage cell line RAW 264.7. As a results, lyoniside decreased the level of nitric oxide and IL-6 in concentration dose dependent manner in RAW 264.7 cells.
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