Construction of Methanol-Sensing Escherichia coli by the Introduction of a Paracoccus denitrificans MxaY-Based Chimeric Two-Component System
2017
Irisappan Ganesh, University of Ulsan, Ulsan, Republic of Korea | Selvamani Vidhya, University of Ulsan, Ulsan, Republic of Korea | Gyeong Tae Eom, University of Ulsan, Ulsan, Republic of Korea | Soon Ho Hong, University of Ulsan, Ulsan, Republic of Korea
Escherichia coli was engineered to sense methanol by employing a chimeric two-component system (TCS) strategy. A chimeric MxaY/EnvZ (MxaYZ) TCS was constructed by fusing the Paracoccus denitrificans MxaY with the E. coli EnvZ. Real-time quantitative PCR analysis and GFP-based fluorescence analysis showed maximum transcription of ompC and the fluorescence at 0.01% of methanol, respectively. These results suggested that E. coli was successfully engineered to sense methanol by the introduction of chimeric MxaYZ. By using this strategy, various chimeric TCS-based bacterial biosensors can be constructed and used for the development of biochemical-producing recombinant microorganisms.
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