Thermoresistant properties of bacterioferritin comigratory protein against high temperature stress in Schizosaccharomyces pombe
2016
Ryu, I.W., Kangwon National University, Chuncheon, Republic of Korea | Lee, S.H., Kangwon National University, Chuncheon, Republic of Korea | Lim, H.W., Shebah Biotech Inc., G-Tech Village, Chuncheon, Republic of Korea | Ahn, K., Baekseok Culture University, Cheonan, Republic of K orea | Park, K., Kangwon National University, Chuncheon, Republic of Korea | Sa, J.H., Gangwon Institute of Health and Environment, Chuncheon, Republic of Korea | Jeong, K.J., Gangwon Institute of Health and Environment, Chuncheon, Republic of Korea | Lim, C.J., Shebah Biotech Inc., G-Tech Village, Chuncheon, Republic of Korea | Kim, K., Kangwon National University, Chuncheon, Republic of Korea
The Schizosaccharomyces pombe structural gene encoding bacterioferritin comigratory protein (BCP) was previously cloned using the shuttle vector pRS316 to generate the BCP-overexpressing plasmid pBCP10. The present work aimed to evaluate the thermoresistant properties of BCP against high temperature stress using the plasmid pBCP10. When the S. pombe cells were grown to the early exponential phase and shifted from 30Celsius to 37Celsius or 42Celsius, the S. pombe cells harboring pBCP10 grew significantly more at both 37Celsius and 42Celsius than the vector control cells. After 6 h of the shifting to higher incubation temperatures, they contained the lower reactive oxygen species (ROS) and nitrite content, an index of nitric oxide (NO), than the vector control cells. After the temperature shifts, total glutathione (GSH) content and total superoxide dismutase (SOD) activities were much higher in the S. pombe cells harboring pBCP10 than in the corresponding vector control cells. Taken together, the S. pombe BCP plays a thermoresistant role which might be based upon its ability both to down-regulate ROS and NO levels and to up-regulate antioxidant components, such as total GSH and SOD, and subsequently to maintain thermal stability.
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