Enhancement of Bacillus thuringiensis Cry1Ac and Cry1Ca toxicity against Spodoptera exigua (H'bner) by suppression of a chitin synthase B gene in midgut
2017
Kim, H.S., Andong National University, Andong, Republic of Korea | Noh, S., Andong National University, Andong, Republic of Korea | Park, Y., Andong National University, Andong, Republic of Korea
The chitin synthase B (CHS-B) gene was cloned from midgut of Spodoptera exigua (SeCHS-B). SeCHS-B contains an open reading frame of 4572 nucleotides, encoding a protein of 1523 amino acids with a predicted molecular mass of approximately 174.6 kDa. The SeCHS-B mRNA was expressed in all developmental stages and specifically in the midgut tissue by qRT-PCR analysis. Expression of SeCHS-B gene was suppressed by feeding double-stranded RNA (dsCHS-B, 400 ng/larva) in the third and fifth instar larvae of S. exigua. Suppression of the SeCHS-B gene expression significantly increased 20% and 25% of mortality on pupation and adult emergence of S. exigua. Also, feeding of dsCHS-B significantly enhanced Cry toxin toxicity to third instar larvae as 34% in 10 ㎍ Cry1Ac and 29% in 0.8 ㎍ Cry1Ca at 6 days after treatment. These results suggest that the SeCHS-B gene plays an important role in development of S. exigua and RNA interference may apply to effective pest control with B. thuringiensis.
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