Anti-Oxidant and Anti-Inflammatory Activities of Hot Water Extract Obtained from Geranium thunbergii Using Different Extraction Temperatures and Times
2018
Sung, H.M., Jeonnam Bioindustry Foundation, Naju, Republic of Korea | Seo, Y.S., Jeonnam Bioindustry Foundation, Naju, Republic of Korea | Yang, E.J., Jeonnam Bioindustry Foundation, Naju, Republic of Korea
In this study, we investigated major flavonoids in the hot water extract from Geranium thunbergii. The results of high-performance liquid chromatography showed that hot water extract from G. thunbergii contained 2 flavonoids, of which the major one was kaempferol followed by quercetin. Also, G. thunbergii was extracted at different temperatures (50℃, 70℃, and 90℃) and times (1, 2, 3, and 4 h), and the hot water extracts were evaluated for extraction yield, kaempferol content, anti-oxidant activity, and anti-inflammatory activity. The extraction yield, kaempferol content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities and nitric oxide (NO) inhibitory activity increased as the extraction temperature increased. In the evaluation of the extraction time of G. thunbergii, the content of kaempferol was highest after 2 h of extraction, but the extraction yield, DPPH and ABTS radical scavenging activities, as well as the NO inhibitory activity were not different after more than 2 h of extraction. We further investigated the effects of hot water extract from G. thunbergii with different extraction times on the expression of pro-inflammatory genes such as iNOS, COX-2, IL-1β, and IL-6 in lipopolysaccharides-stimulated RAW 264.7 cells. At 10 μg/mL, hot water extract with an extraction time of 2 h showed the highest decrease in expression of pro-inflammatory genes among hot water extracts with different extraction times. Based on these results, we suggest that the optimum hot water extract conditions of G. thunbergii are 90KR2018004420 for 2 h and that hot water extract from G. thunbergii has the potential for use as an anti-oxidant and anti-inflammatory agents.
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