Identification of scale insects on coconut and associated crops using 28s gene
2016
Laquinta, J.F. | Rubico, E.C. | Latina, R.A. | Sapin, G.D. | Guerrero, M.S. | Caolli, B.L.
Previous studies using molecular markers identified at least two armored scale insect species, namely, Aspidiotus destructor Signoret and As. rigidus Reyne, in the outbreak areas. At present, verification of armored scale insect species is being conducted in highly-infested coconut areas in such as CALABARZON [Cavite, Laguna, Batangas, Rizal, Quezon], Bataan, Zamboanga and Basilan [Philippines]. Samples were also collected from coconut-associated crops, which show symptoms of scale insect damage. DNA extraction was done through withdrawal of the scale insect exudates. The 28S nucleotide region was then amplified and used as DNA barcode. BLAST hit results revealed four species (98-100% identity) of armored scale insects specially, Abgrallaspis cyanophylli (Signoret). As, destructor, As. rigidus, and As. excisus Green, Soft scale insects namely, Coccus hesperidium L., C. formicarri (Green), Paralecanium expansum (Green) and species relataed to Eriococcus (?) spuruis (Modeer) were identified to infest coconut. Other scale insect species from different hosts were also identified as As. cryptomeriae Kuwana and Parasaissetia nigra (Nietner) and species related to Unaspis euonymi Comstock and Chionapsi wistariae (Cooley). Armored scale insect species found on coconut varieties in Philippine Coconut Authority - Zamboanga Research Center, on the other hand, shared 98% to 100% identity with As. destructor. Moreover, scale insect species collected from different palm species in Basilan and identified as As. rigidus, Ab. cyanophyll, C. formicarii, and P. expansum, apperaed to be few and infrequently observed, and are found on coconut-associated crops such as coffee, lanzones, mangosteen and banana.
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