Успостављање / увођење брзог и ефикасног in vitro система размножавања Paulownia elongata / Establishment / introduction of fast and efficient in vitro propagation system of Paulownia elongata
2015
Вујовић, Татјана (Институт за воћарство, Чачак (Србија)) | Ружић, Ђурђина (Институт за воћарство, Чачак (Србија)) | Церовић, Радосав (?Иновациони центар ТМФ, Београд (Србија))
A successful protocol for micropropagation of Paulownia elongata was developed. Shoot tips stripped from planted nursery trees, as well as single-node green cuttings are recommended as initial explants. The highest multiplication index was obtained on Murashige and Skoog medium (MS) (1962) supplemented with 4 mg l-1 BA and 0.2 mg l-1 NAA (1 : 4.67), while the highest length of axial shoot was obtained on MS medium with 4 mg l-1 BA and 0.1 mg l-1 IBA (2.63 cm). Percentage of rooting was 100% with all used combination of auxins, but the highest number of roots was obtained on MS ½ with combination of 0.5 mg l-1 NAA, 0.5 mg l-1 IBA and 0.1 mg l-1 GA3 (12.5). Under the ‘mist’ irrigation the acclimatization was 100%. The developed protocol encourages the use of micropropagation for propagation and spreading of this important forest species in our country.
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