Effect of hnRNP-like protein THO4 on growth and mRNA export in fission yeast
2018
Park, J.H., Sungshin Women's University, Seoul, Republic of Korea | Lee, S., Sungshin Women's University, Seoul, Republic of Korea | Yoon, J.H., Sungshin Women's University, Seoul, Republic of Korea
The evolutionally conserved TREX complex member, Yra1/ALY, belongs to the REF (RNA and export factor binding proteins) family of hnRNP-like proteins, which has been implicated in multiple processes including transcription, nuclear RNA stability, and mRNA export. Fission yeast, Schizosaccharomyces pombe, genome encodes two members of REF proteins. In addition to Mlo3 known previously as an mRNA export factor, there is the other REF protein, Tho4, which is predicted as a component of THO complex. Here we showed that deletion of tho4 (SPBC106.12c) gene does not inhibit both growth and nuclear mRNA export. However, overexpression of tho4 displays growth retardation and slight accumulation of poly(A)+ RNA in the nucleus. Neither Δtho4Δmlo3 nor Δtho4 Δmex67 double mutants exhibit additive growth defect. Moreover, yeast two-hybrid and co-immunoprecipitation analysis did not show that the Tho4 protein interacted with any members of TREX complex and mRNA export factor Rae1. Contrary to expectation, these observations support that the S. pombe Tho4 is not a component of TREX complex, and not directly involved in bulk mRNA export from the nucleus.
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