Expression, glycosylation, and function of an anti‑rabies virus monoclonal antibody in tobacco and Arabidopsis plants
2018
Song, I.C., Chung-Ang University, Seoul, Republic of Korea | Park, S.A., Chung-Ang University, Seoul, Republic of Korea | Han, D., Korea Centers for Disease Control and Prevention, Osong, Republic of Korea | Lee, H.K., Korea Centers for Disease Control and Prevention, Osong, Republic of Korea | An, H.J., Chungnam National University, Daejeon, Republic of Korea | Ko, K., Chung-Ang University, Seoul, Republic of Korea
Plants have emerged as one of the most attractive systems for producing human therapeutic proteins against viral diseases. These include diagnostic reagents, vaccines, and antibodies. This process is known as molecular biofarming. The objective of this study was to develop and evaluate tobacco and Arabidopsis as plant platforms for producing human anti-rabies monoclonal antibody (mAb). Both tobacco and Arabidopsis transgenic plants were generated by Agrobacterium-mediated transformation. Purifcation of mAb SO57K from each plant was performed with ammonium sulfate-mediated precipitation and protein A afnity columns. SDS-PAGE analysis showed that the purity of mAb SO57K obtained from each transgenic plant was similar, whereas Arabidopsis showed approximately twofold greater protein expression than tobacco. The N-glycosylation was not signifcantly diferent between proteins from the two plant species, with both showing oligo-mannose glycan structures. The mAbs SO57 derived from both the model plants had similar neutralizing efcacy against target virus strain CVS-11. Taken together, tobacco and Arabidopsis are both promising platforms for producing a human anti-rabies mAb.
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