Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples
2018
Amudha Jayaprakasan, SASTRA University, Thanjavur, India | Amarnath Thangavel, SASTRA University, Thanjavur, India | Lakshmishri Ramachandra Bhat, SASTRA University, Thanjavur, India | Manju Bhargavi Gumpu, SASTRA University, Thanjavur, India | Noel Nesakumar, SASTRA University, Thanjavur, India | K. Jayanth Babu, SASTRA University, Thanjavur, India | Srinivasan Vedantham, SASTRA University, Thanjavur, India | John Bosco Balaguru Rayappan, SASTRA University, Thanjavur, India
Increased consumption of fried foods such as grilled chicken contains elevated levels of methylglyoxal (MG), which is associated with diabetes mellitus. Hence, in this work, glyoxalase 1(GLO 1) based, zinc oxide (ZnO) flakes interfaced mediator free electrochemical biosensor was developed to detect MG in grilled chicken. ZnO flakes were synthesized by direct precipitation method. X-ray diffractometer and field emission scanning electron microscope were used to study the structural and morphological characteristics of ZnO flakes. The immobilization of GLO 1 on Pt/ZnO flakes modified electrode was confirmed by Fourier transform infrared spectroscopy. Cyclic voltammetric and amperometric studies were carried out using Pt/ZnO flakes/GLO 1 working electrode. The developed biosensor exhibited linear range of 0.6-2.0 μM, sensitivity of 0.281 lA lM-¹ , LOD of 9 nM with a response time of \\4 s and shelf life of 18 days (89%).
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