Natural medium for growing endophytic bacteria from Solanaceae
2016
Purnawati, A. | Harijani, W.S. | Windriyanti, W.
Endophytic bacteria are important microorganisms that are potential biocontrol agents for many pathogens. Its growth requires the use of semisynthetic or synthetic medium, the ingredients of which make it difficult for farmers to use in the field. It is also expensive for propagation as biocontrol agents. The study focused on identifying natural ingredients like soybean, chicken broth, egg, worms, snail, and sorghum, which are easy to obtain by farmers. The factors were arranged in completely randomized factorial design for four isolates of endophytic bacteria from solanaceae in Malang, Indonesia, and five natural ingredients for the medium-corn flour, soybean flour, sorghum flour, snail flour, and worm flour. Result of the study showed that the bacteria grew in all of the media used. There is no difference in the incubation period, color, shape, and surface colony. However, the population in the snail flour medium at 10 sup 7 cfu/mL is the highest (7.5x10 sup 6 cfu/mL), thus, the best medium for growing endophytic bacteria.
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