Caracterización y mapeo fino de QTLs implicados en la domesticación de melón (Cucumis melo L.)
2018
Riahi, Ch.
Melon (Cucumis melo L.) is an economically important vegetable species belonging to the Cucurbitaceae family. This species has been cultivated since more than 4000 years ago. Its domestication has not been investigated in detail and the genetic control of domestication-related traits is still poorly understood. The work carried out in this Master's Thesis aims to characterize QTLs involved in the genetic control of two domestication-related traits: pulp content and fruit weight, in mapping populations derived from the cross between the Western elite cultivar "Piel de Sapo" and the Pakistan wild accession "Trigonus", as well as to fine map the fruit weight QTL fwqt8.1 previously identified in chromosome VIII. Different families of advanced introgression lines were analyzed in which an epistatic interaction between QTL located in chromosomes VI and XI, inducing a drastic decrease in pulp content in the presence of the TRI allele in both chromosomes, was revealed. For the fine mapping of fwqt8.1, recombinant introgression lines were studied during the 2017 campaign and the fruit weight QTL position was narrowed down to approximately 700 Kb in a first mapping approach. Then, after the analysis of new recombinant families in the 2018 campaign, the QTL region was reduced to 360 Kb between the CMPSNP97 and CMPSNP44 markers. To fine map the chromosomal position of fwqt8.1, thirty-three recombinant plants were generated. The recombinant events were determined by saturating the region with SNP markers, designed in this work, distributed regularly throughout the target region of the QTL, allow a better resolution mapping in future work
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