Cloning and molecular characterization of chalcone synthase gene from mulberry (Morus alba L.)
2017
Calumpang, C.L.F. | Laurena, A.C.
Two members of the chalcone synthase (CHS) enzyme superfamily of type 3 polyketide synthases (PKSs) are CHS and resveratrol synthase, which synthase flavonoids and resveratrol, respectively, and exhibit health and anti-fungal properties. This study aimed to clone, sequence and analyze partial CHS gene sequences from mulberry (M. alba L.) leaves using designed and published primers. CHS genes were isolated and cloned from mulberry genomic DNA through PCR-based methods using primers based on conserved regions of members of the CHS super family of type 3 PKSs. The 584-bp PCR amplicon generated two CHS clones having high sequence identity (80%) with CHS sequences from other plant sources. Phylogenetic analysis with other plant sources exhibited clustering of both sequences together with other angiosperm CHS sequences, specifically with decots, which is consistent with mulberry classification. The CHS partial sequence corresponded to a 195 amino acid deduced protein, which exhibited several predicted conserved domains, including enzyme activity site and dimer interface. Catalytic and conserved amino acid residues among CHS enzymes were present. Three-dimensional homology modeling predicted a homodimeric protein with high homology to alfalfa CHS crystal structure.
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