SSR markers for mango (Mangifera indica L.) cultivar identification and genetic characterization
2017
Sales, E.K. | Butardo, N.G.
The study identified SSR markers that can discriminate the different mango cultivars in the Philippines. It was conducted to select markers that are specific for Philippine Carabao Mango that can be used as a diagnostic kit for true to type variety. Two hundred mango samples were collected and evaluated while 49 SSR primers were screened for their utility in assessing the identity of the said mango cultivars. Out of these 49 primers, 29 primers were selected for further analysis. Based on the generated molecular data, e.g. allele size, fragments amplified, polymorphic information content (PIC) and powers of discrimination, the 29 primers were able to discriminate carabao cultivars over that of other cultivars sampled. An arbitrary genotypic assignment was also established to further show the differences among the cultivars/samples evaluated. The results show that the primers selected can be used to validate the authenticity of the carabao strain as labeled.
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