In-vitro selection of pineapple cv. 'Queen' with resistance to culture filtrate of Phytophthora cinnnamomi Rands
2014
Valencia, L, DC. | Pascual, C.B. | Delfin E.F.
Laboratory and growth room studies were conducted to develop a tissue culture medium for pineapple callus induction and in-vitro selection of pineapple cv. 'Queen' with resistance to culture filtrate of Phytopthora cinnamomi Rands. Basal Murashiege and Skoog's (MS) medium supplemented with 10 mg/L 4-amino -3.5, 6-trichloro-2-pyridine carboxylic acid (picloram) was found to be the most effective for callus induction after 4wk of culturing the explant in the medium. High frequency of regeneration of calli into plantlets was observed in MS medium supplemented with 5 muM benzene amino purine (BAP) and 1muM gibberellic acid (GA3). The Phytophthora cinnamomi Rands isolate used in the study was isolated from Phtophthora heart rot infected pineapple from Calauan, Laguna, Philippines. Inoculation of pineapple calli with different concentrations of P. cinnamomi crude filtrate showed that the toxic effects of the crude filtrate on the treated calli decreased with the reduction in filtrate concentration. Calli resistant to P. cinnamomi crude filtrate were selected and regenerated into plantlets after 3 cycles of selection.
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