In vitro root and shoot formation of Rosa centifolia using plant growth regulators
2018
Akhtar, G. | Muhammad Jafar Jaskani | Sajjad, Y. | Akram , A. | Farooq, A. | Rasool. G.
Rosa centifolia is one of the most economically important floriculture crops that is grown and used for oil extract which has various applications in pharmaceutical industries. It is commercially propagated through cuttings, but low rooting percentage has always been a problem since rose species are difficult to root. To address this problem, a protocol was developed to meet the growing demands for R. centifolia through in vitro propagation technique. Nodal segments were used as an explant and were cultured in a MS medium supplemented with different concentrations (0.2, 0.4, 0.6 and 0.8muM) of Benzylaminopurine (BAP) and Kinetin (KIN), alone and in combination of both, for induction of shoots. For root induction, different concentrations (0.2, 0.4, and 0.6muM) of Indole Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA) were used in a 1/2 strength MS medium. BAP at 0.2muM concentration produced the highest number of shoots (1.93) and longest shoot elongation (5.55cm) vis-a-vis other concentrations. Moreover, minimum number of days (10.2) to shoot induction was observed at (0.4muM of BAP in combination with 0.2muM of KIN. On the other hand, IBA at 0.4muM had the minimum number of days (10.07) to produce roots, highest number of roots (2.47), and longest root elongation (4.21cm) among other concentrations. Thus, it is concluded that BAP at 0.2muM and IBA at 0.4muM concentration are effective for shoot and root formation of R. centifolia, respectively.
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