Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia
2018
ojiewo chris | amede tilahun | fikre asnake | adem makka | degefu tulu | wolde-meskel endalkachew
Pigeon pea (Cajanus cajan (L.) Millsp.) is an important protein source grown in several tropical and subtropicalcountries, and is considered a multi-purpose plant that is resistant to the conditions wheredrought and salinity is a common phenomenon. The aim of this study was to evaluate the diversity ofrhizobial isolates obtained from root nodules of pigeon pea plants grown in central and southernEthiopia. A total of 116 nitrogen-fixing rhizobial strains were isolated. The bacterial isolates werecharacterized by 91 phenotypic traits including cultural characteristics, intrinsic antibiotic and heavymetal resistance, salt, pH and incubation temperature tolerance, and carbon and nitrogen sourcesutilization ability. Preliminary symbiotic properties of the isolates were also evaluated. The isolateswere compared with seven reference strains of rhizobia by application of the unweighted pair groupmethod with arithmetic means (UPGMA) using NTSYSpc Version 2.1 software program. Thedendrogram constructed from cluster analysis of 91 phenotypic traits, grouped them into six clustersand eight un-clustered positions at 80% relative similarity. Cluster I contained 83% of the test isolatesthat were grouped together with the reference strains Bradyrhizobium japonicum (HAMBI 2314T) andBradyrhizobium elkanii (LMG 6164), suggesting that pigeon pea is commonly nodulated bybradyrhizobia. Results from symbiotic effectiveness test revealed that majority of the isolates werefound to be effective. Generally, this investigation demonstrated that rhizobial population nodulating C.cajan on the study area were phenotypically diverse and symbiotically effective. Furthermore, the resultindicates the existence of strains in the collection, which can tolerate environmental stresses, thus canbe developed into inoculant for pigeon pea inoculation and production in Ethiopia and beyond
Afficher plus [+] Moins [-]Degefu Tulu et al., 'Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia', African Journal of Biotechnology, vol. 17(6), pp.167-177, Academic Journals, 2018
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