Determination of ellagic acid in raspberry seeds
2018
Marić, Boško (https://orcid.org/0000-0003-4264-8086) | Bodroža-Solarov, Marija (https://orcid.org/0000-0001-8151-6276) | Ilić, Nebojša (https://orcid.org/0000-0002-4187-1916) | Abramović, Biljana | Krulj, Jelena (https://orcid.org/0000-0001-7348-3961) | Kojić, Jovana (https://orcid.org/0000-0002-8816-9892) | Milenković, Slobodan
Large amount of raspberries used in the production of raspberry products, leave large amount of raspberry seeds which are considered as a waste or by-product. These seeds are rich in bioactive compounds and contain a lot of antioxidants such as phenols, flavonoids, antocyanins, vitamins, and other compounds beneficial for human health. One of these compounds, ellagic acid is polyphenolic compound which is well known for its biological activity. It has antimicrobial, antimutagenic, antioxidant, and anti-inflammatory activity. The main objective of this study was to determine ellagic acid content in raspberry seeds pellet left from oil extraction. Five different methods were used for oil extraction such as Soxhlet extraction, extraction with methylene chloride, extraction with chloroform, cold pressed extraction and supercritical fluid extraction (SFE). HPLC coupled with DAD detector was used to determine ellagic acid content. The obtained results show that the largest amount of ellagic acid was determined in pellet after cold pressed extraction. Also, the results show that raspberry seeds are rich source of ellagic acid. Knowing this and because of the high production of raspberries in our geographic area and the amount of waste in the production of raspberry products, raspberry seeds could be used as a significant source of ellagic acid.
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