A comparison of two methods for determination of HMF in honey: HPLC method versus spectrophotometric method
2019
Tasić, Aleksandra (https://orcid.org/0000-0002-8361-5697) | Mitrović, Tijana | Pavlović, Marija (https://orcid.org/0000-0002-4854-8628) | Kureljušić, Jasna (https://orcid.org/0000-0002-9366-1857)
In this paper the results of control of the quality of honey on the market of the Republic of Serbia are given by controlling the quantity of one of the most important control parameters, which is the content of 5-hydroxymethyl-2- furfural (HMF). Analysis of honey samples was carried out using two methods: high performance liquid chromatography (HPLC) and spectrophotometry method. It is important to note that the quality of honey, and in this connection, the HMF content in the honey, is influenced by a number of factors, such as processing conditions, the manner and period of packaging of honey, as well as the place of storage up to its consumption. The analysis of the results shows a large percentage of honey samples that had a permissible amount of HMF, but in the analysis there was a small number of samples that did not satisfy the mentioned quality parameter. The aim of study was developing two methods for determination of HMF; HPLC and spectrophotometric method and comparing the results obtained for the certified reference material. The results of the test showed good agreement of the results and high accuracy of the results obtained using both methods for determining HMF in the honey.
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