Evaluation of caffeine, vitamins and taurine in energy drinks
2019
Lage-Yusty, María Asunción | Villar-Blanco, Lorena | López-Hernandez, Julia
In this work, a rapid and inexpensive method of high performance liquid chromatography (HPLC) with photodiode array and fluorescence detection was developed to determine ascorbic acid, pyridoxine, thiamine, riboflavin and caffeine simultaneously in energy drinks, using spectrophotometric detection at different wavelengths in a relatively short time. Taurine determination was carried out by HPLC with ultraviolet-visible detector using pre‐column deri- vatization with 2,4-dinitrofluorobenzene. Ultraviolet-visible absorption spectra were used to confirm the identity and purity of pyridoxine, caffeine and riboflavin. Limit of detection and limit of quantification ranged from 0.002 µg·ml-1 to 2.04 µg·ml-1 and from 0.008 µg·ml-1 to 2.12 µg·ml-1, respectively. Precision (relative standard deviation) was between 1.9 % and 7.8 %. In the energy drinks, caffeine concentration was found to be 252–304 mg·l-1, taurine 2 180–2 750 mg·l-1, pyridoxine 13.0–39.8 mg·l-1 and riboflavin from not detectable to 16 mg·l-1. Ascorbic acid and thiamin were not detected. Labelling of the beverages regarding vitamins, caffeine and taurine contents did not correspond to results of analysis of samples.
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