Production of silver nanoparticles (AgNPs) by certain bacterial strains and their characterization

Abo-State, M.A.M.; Partila, A.M.

Production of silver nanoparticles (AgNPs) by certain bacterial strains and their characterization

2018

Abo-State, M.A.M. | Partila, A.M.

Development of biologically inspired experimental processes for the biosynthesis ofnanoparticles (NPs) is an evolving important branch of nanotechnology. In the present work, we studiedthe potential of four bacterial species for extracellular production of nanosilver (AgNPs) from 3 mMconcentration of silver nitrate (AgNO3) after incubation for 4h at 85°C. Biosynthesized AgNPs werecharacterized by using different methods such as; UV/vis spectroscopy, Transmission electronmicroscope (TEM), X-Ray Diffraction (XRD) and Fourier Transform Infra-red (FTIR) spectroscopy.Results of UV–vis spectroscopy showed maximum absorption at 401-432 nm, which represents thecharacteristic surface plasmon resonance of AgNPs. TEM demonstrated that the size range of these NPsranged approximately from 7.8- 13.4 nm. Representing the XRD pattern obtained for the AgNPs, anumber of Fcc structures of silver Bragg reflections corresponding to (100), (110), (111), (200) and (220)planes were observed. FTIR results recorded a downward shift of absorption bands between 400–4000cm−1indicating the formation of AgNPs. Finally we concluded that the extracellular biosynthesis ofAgNPs by the four bacterial species; Ochrobactrum sp. (MAM-C9), Achromobacter xylosoxidans(MAM-29), Pseusomonas aeruginosa (MAM-42) and Bacillus cereus (MAM-I.11) were confirmed. Thisstudy recorded that bacterial biosynthesis of AgNPs is useful to avoid adverse effects of chemical andphysical methods that are non-suitable for medical applications.

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