A semiselective medium for the isolation of Acidovorax valerianellae from soil and plant debris
2007
GRONDEAU, Catherine | Manceau, Charles | SAMSON, Régine
Bacterial black spot of corn-salad (Valerianella locusta) caused by Acidovorax valerianellae appeared in France in the beginning of the 1990s. The disease is now widespread in the main area of corn-salad production and is responsible for high depreciation and significant economic losses every year. A semiselective medium is described for the detection of the bacterium in various environments and to identify primary sources of inoculum. The medium TSAV (tryptic soy broth, 3 g L-1, agar 15 g L-1, 5-fluorouracyl 5 mg L-1, novobiocin 5 mg L-1, propiconazole 5 mg L-1) increased chances of obtaining cultures of A. valerianellae from naturally infested soil and from root-debris. Using TSAV, the pathogen was detected in root-debris up to 39 days after the harvest of a diseased crop. As only a few days usually separates harvest from the next sowing date, soil is likely to be an important source of inoculum for the next crop
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