Existence of two membrane-bound acetylcholinesterase in the honeybee head
2007
Badiou, Alexandra | Brunet, Jean-Luc | Belzunces, Luc
Two acetylcholinesterase (EC 3.1.1.7) membrane forms AChEm1 and AChEm2, have been characterised in the honey bee head. They can be differentiated by their ionic properties: AChEm1 is eluted at 220 mM NaCl whereas AChEm2 is eluted at 350 mM NaCl in anion exchange chromatography. They also present different thermal stabilities. Previous processing such as sedimentation, phase separation, and extraction procedures do not affect the presence of the two forms. Unlike AChEm1, AChEm2 presents reversible chromatographic elution properties, with a shift between 350 to 220 mM NaCl, depending on detergent conditions. Purification by affinity chromatography does not abolish the shift of the AChEm2 elution. The similar chromatographic behaviour of soluble AChE strongly suggests that the occurrence of the two membrane forms is not due to the membrane anchor. The two forms have similar sensitivities to eserine and BW284C51. They exhibit similar electrophoretic mobilities and present molecular masses of 66 kDa in SDS-PAGE and a sensitivity to phosphatidylinositol-specific phospholipase C in non-denaturing conditions, thus revealing the presence of a glycosyl-phosphatidylinositol anchor. We assume that bee AChE occurs in two distinct conformational states whose AChEm2 apparent state is reversibly modulated by the Triton X-100 detergent into AChEm1
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