Involvement of NRAMP1 from Arabidopsis thaliana in iron transport.

Curie, Catherine; Alonso, Jose M.; Le Jean, Marie; Ecker, Joseph R.; Briat, Jean-François,

Involvement of NRAMP1 from Arabidopsis thaliana in iron transport.

2000

Curie, Catherine | Alonso, Jose M. | Le Jean, Marie | Ecker, Joseph R. | Briat, Jean-François,

Nramp genes code for a widely distributed class of proteins involved in a variety of processes, ranging from the control of susceptibility to bacterial infection in mammalian cells and taste behaviour in Drosophila to manganese uptake in yeast. Some of the NRAMP proteins in mammals and in yeast are capable of transporting metal ions, including iron. In plants, iron transport was shown to require a reduction/Fe(II) transport system. In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes. Here we report the sequence of five NRAMP proteins from A. thaliana. Sequence comparison suggests that there are two classes of NRAMP proteins in plants: A. thaliana (At) NRAMP1 and Oriza sativa (Os) NRAMP1 and 3 (two rice isologues) represent one class, and AtNRAMP2-5 and OsNRAMP2 the other. AtNramp1 and OsNramp1 are able to complement the fet3fet4 yeast mutant defective both in low- and high-affinity iron transports, whereas AtNramp2 and OsNramp2 fail to do so. In addition, AtNramp1 transcript, but not AtNramp2 transcript, accumulates in response to iron deficiency in roots but not in leaves. Finally, overexpression of AtNramp1 in transgenic A. thaliana plants leads to an increase in plant resistance to toxic iron concentration. Taken together, these results demonstrate that AtNramp1 participates in the control of iron homoeostasis in plants.

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Mots clés AGROVOC

plant

Informations bibliographiques

D'autres materias

Mesh : amino acid sequence; Mesh : genes; Genetically modified; Mesh : homeostasis; Mesh : genetic complementation test; Mesh : multigene family; Mesh : iron; Mesh : plant proteins; Mesh : molecular sequence data; Mesh : phylogeny; Mesh : gene expression regulation; Mesh : carrier proteins; Mesh : oryza; Molecular; Mesh : membrane transport proteins; Mesh : mutation; Messenger; Fungal; Mesh : conserved sequence; Mesh : biological transport; Mesh : sequence alignment; Mesh : plants; Mesh : cloning; Mesh : rna; Mesh : saccharomyces cerevisiae; Mesh : arabidopsis; Mesh : cation transport proteins; Mesh : membrane proteins

Langue

anglais

Licence

http://creativecommons.org/licenses/by-nc-nd/

Type

Art

Source

Portland Press

Sur AGRIS depuis: 2021-03-15

Format: AGRIS AP

Fournisseur de données

Cette notice bibliographique a été fournie par Institut national de la recherche agronomique