Partial purification and molecular weight determination of cellulase from Bacillus cereus
2016
Nema, N. | Alamir, L. | Mohammad, M.
This work describes the partial purification and molecular weight determination of cellulase enzymes produced by submerged fermentation using Bacillus cereus. The enzyme was purified using phenyl-sepharose and sephadex G-100 columns up to 34.6 fold with a specific activity reaching 0.104 IU/mg. The molecular weight of the purified enzyme was determined to be 16.9 kDa by means of SDS-PAGE. This molecular weight is comparable with that of other low molecular mass cellulases produced by Bacillus spp. This finding emphasizes that cellulase produced from Bacillus cereus through submerged fermentation using corn husks belongs to a group of low molecular cellulases.
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