Xylanase production by a new Bacillus pumilus SY30A under solid state fermentation and its application in oil palm biomass pulp bleaching
2016
Akeed, Yasser | Ammouneh, Hassan | Audi, Ruba | Bakri, Yasser | Harba, Muhanad | Leh, Cheu Peng
In the current study, the production of extracellular xylanase by Bacillus pumilus SY30A was investigated in a solid state fermentation. The effects of temperature and pH on xylanase production were tested. The best physical parameters observed forthe enzyme production by B. pumilus SY30A were 37 °C and pH 9 respectively. Results showed that wheat bran was found to support the highest yield of xylanase among various carbon sources tested. The maximum enzyme production was obtained 1438 IU/g after 72h of fermentation. The crude xylanase was then used to pretreat the unbleached oil palm soda-anthraquinone pulp. Results showed that with only 20 U of B. pumilus SY30A crude extract xylanase of 55 °C for 1 hour, decreased the kappa number of 43% while increased the pulp viscosity of 10%. This preliminary study on paper pulp bleaching disclosed the potential use of B. pumilus SY30A xylanase as biobleaching agent, which is also a more environmental sustainable method in comparison to those conventional chemical bleachingmethod.
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