Gender identification of domesticated chicken using a PCR-based method
2010
Teh, Richard Swee Aun | Jayaraj Vijaya Kumaran
The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently,the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR targeting the CHD-W and CHD-Z genes in the chicken’s chromosome Z and chromosome W, respectively. The results of this study showed that the PCR based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method.
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