Bioactive compounds and antioxidant activities of Camellia sinensis var. assamica in different leave maturity from Northern Thailand
2016
Nimkamnerd, J. | Ruengnet, P. | Nantitanon, W. | Dorkbuakaew, N. | Pradmeeteekul, P. | Thitipramote, N.
The aim of this study was to compare the bioactive compounds [total phenolic content (TPC), total flavonoid content (TFC), and proanthocyanidin content] and antioxidant activities [DPPH, ABTS and FRAP activities] of 3 different maturity of tea leaves, shoot (first 1-3 leaves), young leaves (4-6 leaves) and mature leaves (1-3 from bottom tea shrub) by using different solvents [hot DI water (80OC), DI water, 70% ethanol and 70% acetone] for extraction. Results showedthat the highest extractable yields were found on the shoot tea with hot DI water extraction (21.33 ± 9.15% dry basis). Shoot tea with ethanol extraction composed of high amount of TPC (65.26 mg trolox equivalent antioxidant capacity TEAC/mg sample). Shoot and youngtea leaves in ethanol extract tended to have high proanthocyanidin content (35.94 and 32.15 mg epicatechin equivalent (ECE)/g sample, respectively) and DPPH free-radical scavenging activity (31.39 and 29.93 mg TEAC/g sample, respectively). Mature leaves by 70% acetone extracted showed obviously high amount of flavonoid content (57.39 mg quercetin equivalent (QE)/g sample) and FRAP activity (1.938 mg TEAC/mg sample). Therefore, the young and mature tea leaves that did not be used for tea processing, may be used as an alternative sourceof natural antioxidant for cosmetic and other products.
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